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KMID : 0364820150510020141
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Korean Journal of Microbiology
2015 Volume.51 No. 2 p.141 ~ p.147
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lsolation and identification of β-glucosidase producing halophilic Roseivivax roseus
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Cho Geon-Yeong
Han Song-Ih
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Abstract
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Four halophilic bacteria were isolated from a salt water tank of more than 25% above salinity used for production of salt. HJS1 and HJS6 strains were identified as having β-glucosidase producing capabilities at high salinity. β-Glucosidase produced from these bacterial strains showed the best activity at 56-79 U/ml in NaCl [0-5%], showing the highest β-glucosidase activity at NaCl 3%. A salt tolerant β-glucosidase can maintain at least 75% activity of the enzyme in 0-20% NaCl concentration. The 16S rRNA gene sequences of strains HJS1 and HJS6 shows 99.8% similarity with Roseivivax roseus BH87090T. Those sequences were registered as AB971835 and AB971836 in the NCBI GenBank. DNA-DNA hybridization test revealed that both strains showed 90.1 to 90.3% hybridization values with R. roseus BH87090T, which was the closest phylogenetic neighbor. Major Cellular fatty acids of strains HJS1 and HJS6 were C16：0, C18 : 1 ω7 c, C19:0 cyclo ω8 c and 11 - methyl C18 : 1 and the major quinone was Q-10. Their fatty acid composition and quinone were very similar to Roseivivax roseus BH87090T. Meanwhile, Roseivivax roseus BH87090T did not produce any β-glu cosidase. Based on the molecular and chemotaxonomic properties, strains HJS1 and HJS6 were identified as members of Roseivivax roseus.
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KEYWORD
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Roseivivax roseus, β-glucosidase, halophilic
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